Mode of action of a bacteriocin from Erwinia carotovora II. Degradation of phospholipids in carotovoricin ER-treated cells.

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Abstract
Degradation of macromolecules such as DNA, RNA, protein and phospholipid in carotovoricin Er-treated cells was examined. No significant decrease in acid-insoluble radioactivity of DNA, RNA or protein which were labeled with 3H-thymine, 3H-uracil and 3H-phenylalanine, respectively, could be detected in the carotovoricin Er-treated cells. Marked degradation of phospholipids labeled with 2-3H-glycerol was observed in the cells exposed to the bacteriocin. There was a similar decrease in radioactivity of both 3H and 32P in the lipid fraction double-labeled with 2-3H-glycerol and 32PO43-. This decrease in radioactivity of phospholipids was explained by the release of fatty acid moieties from phospholipids. Phospholipid degradation in carotovoricin Er-treated cells is apparently caused by the action of phospholipase A. The amount of fatty acids released from phospholipids was proportional to the number of cells killed by the bacteriocin, suggesting that phospholipase A activity involved in phospholipid degradation is located in the sensitive cells, not in the bacteriocin.

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