Method for Resolving and Measuring Overlapping Chromatographic Peaks by Use of an On-Line Computer with Limited Storage Capacity

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Abstract
A simple routine for distinguishing and measuring the positions and areas of overlapping peaks comprising a complex chromatographic "envelope" has been developed for a small on-line computer. The degree of peak overlap is such that no minima and only one maximum occur within the bounds of the envelope. This routine determines corrected initial estimates of the Gaussian parameters of the peaks. The routine is currently being used, in conjunction with a program devised to analyze data generated by a chromatographic system, specifically to evaluate data from high-resolution analyzers.

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