Identification of a mammalian gene structurally and functionally related to the CDC25 gene of Saccharomyces cerevisiae.
- 1 August 1992
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (15) , 7100-7104
- https://doi.org/10.1073/pnas.89.15.7100
Abstract
The yeast Saccharomyces cerevisiae CDC25 gene encodes a nucleotide-exchange-factor (NEF) that can convert the inactive GDP-bound state of RAS proteins to an active RAS-GTP complex. CDC25 can activate the yeast RAS proteins as well as the human H-ras protein. CDC25 is a member of a family of yeast genes that likely encode NEFs capable of regulating the RAS-related proteins found in yeast. By aligning the amino acid sequence of CDC25-related gene products we found a number of conserved motifs. Using degenerate oligonucleotides that encode these conserved sequences, we have used polymerase chain reactions to amplify fragments of mouse and human cDNAs related to the yeast CDC25 gene. We show that a chimeric molecule, part mouse and part yeast CDC25, can suppress the loss of CDC25 function in the yeast S. cerevisiae.Keywords
This publication has 46 references indexed in Scilit:
- ERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGFCell, 1991
- Enhancement of the GDP-GTP Exchange of RAS Proteins by the Carboxyl-Terminal Domain of SCD25Science, 1990
- S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating proteinCell, 1990
- A novel membrane factor stimulates guanine nucleotide exchange reaction of ras proteinsFEBS Letters, 1990
- The C-terminal part of a gene partially homologous to CDC25 gene suppresses the cdc25-5 mutation in Saccharomyces cerevisiaeGene, 1989
- ras GENESAnnual Review of Biochemistry, 1987
- The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathwayCell, 1987
- Differential activation of yeast adenylate cyclase by wild type and mutant RAS proteinsCell, 1985
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970