Cooling of Embryonated Eggs to Produce an LD50 for Coccidioides immitis

Abstract
Cooling 8-day embryonated eggs lowered their resistance to C. immitis, making it possible to determine an LD50 for this organism. Massive injections of millions of arthrospores are normally not fatal; however, cooling the embryos for 24 hrs. resulted in an LD50 with an injn. of 3500 arthrospores.

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