Evidence that Detergent-reactivated Interferons are not Renatured
- 1 July 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 32 (1) , 133-137
- https://doi.org/10.1099/0022-1317-32-1-133
Abstract
The sedimentation rate of human leukocyte interferon (HLIF) reactivated from sodium dodecyl sulfate (SDS) solution was studied by glycerol gradient centrifugation and compared to that of native HLIF. Reactivated HLIF consistently sedimented faster than native HLIF, indicating that full recovery of antiviral activity does not require renaturation of the entire interferon molecule.This publication has 2 references indexed in Scilit:
- Examination of the dissociation of multichain proteins in guanidine hydrochloride by membrane osmometryBiochemistry, 1968
- A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein MixturesJournal of Biological Chemistry, 1961