Analysis of Polychlonnated Biphenyls in Fatty Biological Matrixes by On-Line Supercritical Fluid Extraction and Supercritical Fluid Cleanup
Open Access
- 1 July 1995
- journal article
- Published by Oxford University Press (OUP)
- Vol. 78 (4) , 1051-1054
- https://doi.org/10.1093/jaoac/78.4.1051
Abstract
We present a method that combines the extraction of polychlonnated biphenyls (PCBs) and their separation from relatively large quantities of fat in biological matrixes by combined supercritical fluid extraction and separation. Cyano-functionalized silica gel, silica gel, aluminum oxide, Florisil, 3-aminopropyl- functionalized silica gel, and octadecyl-functionalized silica gel were tested for suitability as chromatographic media for separation of PCBs from lipids. Silica gel, 3-aminopropyl-functionalized silica gel, and Florisil adequately separated PCBs from lipids when eluted with supercritical CO2. Florisil allowed both the extraction of PCBs and their separation from lipids in PCB-spiked chicken egg and fish. Two grams of sample containing PCBs at 0.125 μg/g was sufficient for subsequent separations and the low-level analysis required for the more toxic PCB components.Keywords
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