The Selective Blocking of the Polymerization Reaction of Striated Muscle Actin Leading to a Derivative Suitable for Crystallization

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Abstract
The polymerization reaction of rabbit muscle actin was completely inhibited by reaction of 1 amino acid side chain/protein monomer with 5-diazonium-(1H)[14C]tetrazole. A tryptic peptide fingerprint showed a single peptide labeled by the reagent. The peptide was isolated and the labeled amino acid identified by amino acid analysis as Tyr-53. This side chain is not accessible to the reagent in F-actin. The modification is compared to similar inhibitions by other reagents.