Allogeneic Inhibition Between Two Human Cell Lines2

Publisher Website
Google Scholar
Abstract
The indirect immunofluorescence method was used to analyze cell interaction in population mixtures of two cultured human cells, HEp-2 and amnion (RP Am 1), during growth in vitro. A specific antiserum containing antibodies for antigen on amnion cells but none for antigens on HEp-2 cells made it possible to follow the fate of each cell type under optimal conditions of growth and without the use of aggregating agents. Population analysis revealed that inhibition or death of the amnion cells occurred and the degree appeared to depend on factors influencing cell contact. In contrast, the HEp-2 cells were relatively unaffected or markedly stimulated. Cell homogenates of the HEp-2 and RP Am 1 cells did not inhibit either the isogeneic or allogeneic cells. Partial protection of the amnion cells was afforded by exposure to antibody before growth in mixed culture, and the degree showed some correlation with antibody concentration.

This publication has 0 references indexed in Scilit: