SYNTHESIS OF APOLIPOPROTEINS, ALPHAFETOPROTEIN, ALBUMIN, AND TRANSFERRIN BY THE HUMAN-FETAL YOLK SACK AND OTHER FETAL ORGANS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 85  (FEB) , 191-206
Abstract
Fragments of human fetal organs and blood at 5-11 wk of postfertilization development were cultured in radioactive protein precursors. The secreted products were characterized by immunoprecipitation, and by measuring the mobility of the immunoprecipitates on polyacrylamide gels. Secondary human yolk sacks secreted apolipoproteins A1 and B. The synthesis of other serum products by this organ and by the fetal liver and by the fetal intestines was confirmed. Within the yolk sack, the endoderm, the blood cells and the outside epithelium reacted with antibodies against apolipoprotein A1 and transferrin. By metabolic labeling of umbilical cord blood, it was found that blood did not secrete apolipoproteins A1 and B. Blood cells could therefore not be a source of these secreted products.

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