Comparison of the Recovery of Inorganic Iodide by Paper Electrophoresis or Chromatography

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Abstract
Recovery of carrier-free I131-was compared after paper electrophoresis or chromatography in 3 systems: butanol-acetic acid-water (BAW), butanol-ethanol-ammonia (BEA) and butanol-dioxane-ammonia (BDA). Slightly less I131 wasrecovered with any of the 3 chromatographic systems than with electrophoresis. This was caused both by spread of the I131- on the paper and loss by evaporation. Approximately 5% of the I131- was lost by evaporation in any of the 3 chromatography systems. If I127-, serum or thyroid homogenate was added to the carrier-free I131-, recovery was very similar with electrophoresis and any of the chromatography systems, but always slightly better with electrophoresis. It is concluded that paper chromatography with any of the 3 systems used is satisfactory for the quantitative determination of I131- in thyroid homogenates or serum, but care must be taken to dry undigested homogenate for only very short periods before placing the paper strips in the developing tanks. Deiodination of thyroid homogenate during pancreatin digestion appears to be associated with disruption of peptide bonds rather than with loss of iodine from any particular iodoamino acid.