Iron chlorosis and nitrate reductase activity in response to Fe stress and additional nitrate in sorghum

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Abstract
Effect of additional nitrate supply on chlorosis development at different levels of Fe stress was examined in iron efficient and inefficient sorghum cultivars. Nitrate reductase, the enzyme primarily responsible for nitrate reduction was estimated in roots and leaves as affected by Fe stress and additional nitrate. Young leaves showed differences in chlorosis progression at Fe stress levels. Nitrate reductase activity was depressed in roots and young leaves when iron supply was reduced beyond certain level (4/1). Addition of extra nitrate was not able to enhance NR activity when iron supply was lower than ½ level. It was inferred that nitrate utilisation and iron nutrition influence each other and some minimu level of iron supply is necessary for efficient enzyme activity.