The Presence of Leukotriene C4-Binding Sites in Bovine Corpora Lutea of Pregnancy

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Abstract
The presence of binding sites for [3H]leukotriene (LT) C4 in bovine corpora lutea of pregnancy was investigated with quantitative light microscopic autoradiography. Silver grains were found over small (15-20 microns) and large (20-50 microns) luteal cells and arteriolar smooth muscle. Vascular endothelial cells, erythrocytes in arteriolar lumen, and fibroblasts, on the other hand, contained very few or no net grains. The grain distribution over luteal cells and arteriolar smooth muscle was reduced (p less than 0.001) after coincubation with excess unlabeled LTC4 but not with excess unlabeled LTA4, LTB4, LTD4, LTE4, prostaglandin (PG)E2, PGF2 alpha or PGI2. The large luteal cells contained 16.1 net grains per cell, which was 6.4 and 7.0 times the number of specific grains as in small luteal and arteriolar smooth muscle cells, respectively (p less than 0.001). When the net grains were corrected for cell area differences, large luteal cells and arteriole smooth muscle cells contained a similar number of grains-which was two times as many as those found in small luteal cells. These findings suggest that LTC4 can potentially regulate functions of not only luteal cells but also luteal vasculature.
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