Comparison of MRC-5 and U-373MG astrocytoma cells for detection of cytomegalovirus in shell vial centrifugation cultures
- 1 July 1991
- journal article
- research article
- Published by Springer Nature in European Journal of Clinical Microbiology & Infectious Diseases
- Vol. 10 (7) , 569-572
- https://doi.org/10.1007/bf01967276
Abstract
U-373MG astrocytoma cells are susceptible to human cytomegalovirus (CMV) infection and offer the advantage of a continuous cell line for clinical laboratory use. U-373MG to MRC-5 cells for detection of CMV by centrifugation culture were therefore compared. At 20 h, 10 (6.1 %) versus 12 (7.4 %) of 163 clinical specimens were positive for CMV, and at 40 h, 12 (7.4 %) versus 17 (10.4 %) were positive in U-373MG and MRC-5 cells, respectively. Substantial toxicity was found in U-373MG cells (84 %) when inoculated with blood specimens. For detection of CMV in centrifugation culture, MRC-5 cells are superior due both to higher sensitivity and lesser toxicity.Keywords
This publication has 15 references indexed in Scilit:
- Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone and age of MRC-5 monolayers for detection of cytomegalovirusDiagnostic Microbiology and Infectious Disease, 1990
- Increased sensitivity for rapid detection of cytomegalovirus by shell vial centrifugation assay using mink lung cell culturesJournal of Virological Methods, 1989
- Direct detection of cytomegalovirus from bronchoalveolar lavage samples by using a rapid in situ DNA hybridization assayJournal of Clinical Microbiology, 1989
- Cytomegalovirus Infection and Trans-Activation of HIV-1 and HIV-2 LTRs in Human Astrocytoma CellsAIDS Research and Human Retroviruses, 1989
- Rapid detection of cytomegalovirus by tissue culture, centrifugation, and immunofluorescence with a monoclonal antibody to an early nuclear antigenJournal of Virological Methods, 1987
- Comparison of MRC-5 and HFF cells for the identification of cytomegalovirus in centrifugation cultureDiagnostic Microbiology and Infectious Disease, 1987
- Rapid Detection of Human Cytomegalovirus in the Urine of HumansThe Journal of Infectious Diseases, 1985
- Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimensJournal of Clinical Microbiology, 1985
- Comparison of human cytomegalovirus growth in MRC‐5 human fibroblasts, brain, and choroid plexus cells in vitroJournal of Medical Virology, 1981
- Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimensJournal of Clinical Microbiology, 1978