The Yield and Repair of X-Ray-Induced Single-Strand Breaks in the DNA of Escherichia coli K-12 Cells
- 1 October 1976
- journal article
- letter
- Published by JSTOR in Radiation Research
- Vol. 68 (1) , 148-154
- https://doi.org/10.2307/3574542
Abstract
The yield of single-strand breaks present in chromosomal DNA of E. coli cells after aerobic X irradiation was determined using alkaline sucrose gradient techniques. Experimental conditions were used that avoided a centrifugation speed dependence effect on DNA sedimentation and allowed either minimal or complete enzymatic repair to occur. The extent of DNA strand breakage was previously underestimated by factors of 3.4-7.6, depending on the strain and repair conditions used. Technical and interpretive difficulties in the measurement of DNA single-strand breakage using alkaline sucrose gradient techniques are discussed briefly. In these experiments, the initial yield of DNA single-strand breaks was 32.4/genome krad-1 (9.0 eV/break).This publication has 3 references indexed in Scilit:
- R.B.E. of 50 kVp X-rays and 660 keV γ-rays (137Cs) with Respect to the Production of DNA Damage, Repair and Cell-killing inEscherichia ColiK-12International Journal of Radiation Biology, 1975
- Reconstruction in vivo of Irradiated Escherichia coli Deoxyribonucleic Acid; the Rejoining of Broken PiecesNature, 1966
- The anatomy of the T5 bacteriophage DNA moleculeJournal of Molecular Biology, 1966