IMMUNOELECTROPHORETIC DETERMINATION OF TEAR FLUID PROTEINS COLLECTED BY THE SCHIRMER I TEST
- 30 November 1979
- journal article
- research article
- Published by Wiley in Acta Ophthalmologica
- Vol. 57 (6) , 959-967
- https://doi.org/10.1111/j.1755-3768.1979.tb00526.x
Abstract
Using reservoirs containing 50 .mu.l of antigen solution in combination with a micro-modification of the electroimmunoassay a detection limit of 0.05 ng applied in 400 .mu.l corresponding to 0.125 ng/ml was achieved. Human tear fluid eluate from Schrimer paper strips was tested in the assay. The eluation procedure failed to extract all tear proteins as 32 ng were left in the paper. Despite the loss, experiments have shown the method to be suitable for immunological determination of tear proteins collected by the Shirmer I test.Keywords
This publication has 6 references indexed in Scilit:
- A micromodification of the electroimmunoassayAnalytical Biochemistry, 1978
- Immunoelectrophoretic quantitation of trace proteinsJournal of Immunological Methods, 1976
- The origin of some enzymes in tear fluid, determined by comparative investigation with two collection methodsExperimental Eye Research, 1976
- Human Tear LysozymeAmerican Journal of Ophthalmology, 1968
- Standardized Schirmer Tear Test Kit*American Journal of Ophthalmology, 1961
- Studien zur Physiologie und Pathologie der Tränenabsonderung und TränenabfuhrAlbrecht von Graefes Archiv für Ophthalmologie, 1903