Occurrence of Isóenzymes of Glutamine Synthetase in the Alga Chlorella kessleri
- 1 February 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 74 (2) , 204-207
- https://doi.org/10.1104/pp.74.2.204
Abstract
Two forms (GS1 and GS2) of glutamine synthetase have been isolated, separated by ion exchange chromatography, and partly characterized from cells of the green alga Chlorella kessleri. Both forms are present in cells grown autotrophically or heterotrophically on various nitrogen sources, but under all nutritional conditions GS1 was found to be the major isoenzyme present (60-80%). The activity of both isoenzymes was greatest in cells grown under nitrogen-limiting conditions. Both isoenzymes have molecular weights in the range 340 to 350,000 daltons. GS1 was found to have a greater thermostability than GS2: GS1 was stable at 30°C while GS2 lost 95% of its activity in 30 minutes. GS1 was much less sensitive to thiol reactive reagents than GS2.This publication has 21 references indexed in Scilit:
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