B cells are anergic in transgenic mice that express IgM anti‐DNA antibodies

Publisher Website
Google Scholar
Abstract
B lymphocytes in individuals with systemic lupus erythematosus (SLE) secrete pathogenic auto antibodies to DNA which cause clinical nephritis. (NZB x NZW) Fl (BW) female mice also secrete pathogenic anti‐DNA auto antibodies, and therefore are considered to be an animal model of SLE. The rearranged immunoglobulin (Ig) genes that encode an anti‐DNA antibody from a diseased BW mouse have been cloned, and transgenic (Tg) mice have been created by microinjection of these constructs into fertilized eggs from normal mice. As we reported previously, when the construct contains the Cγ2a heavy chain constant (CH) region, the mice spontaneously secrete anti‐DNA IgG and they develop mild nephritis. This demonstrated that the Ig encoded by the transgene is pathogenic. In contrast, here we report that when the construct contains the same anti‐DNA Ig variable (V) regions used previously, along with the Cμ region, the auto reactive B cells are rendered tolerant. Most B cells in the Tg mice express the μ transgene product on their surface, and rearrangement of endogenous light chain genes is partially suppressed. Furthermore, most hybridomas made from Tg B cells secrete IgM anti‐DNA. Despite this, the Tg mice have reduced levels of total serum Ig and they do not secrete anti‐DNA IgM either spontaneously or following immunization with DNA. We conclude that most B cells in the Tg mice have been rendered anergic. Anergy is however reversible in vitro; lipopolysaccharide stimulation of Tg B cells leads to the production of a significant amount of IgM anti‐DNA antibody. The studies demonstrate that in this line of Tg mice on a normal mouse genetic background potentially pathogenic B cells that express a high‐affinity Ig specific for a natural autoantigen are subject to tolerance by induction of anergy.