Molecular genetic analysis of easily accessible breast tumour DNA, purified from tissue left over from hormone receptor measurement
- 1 January 1998
- Vol. 106 (1-6) , 371-377
- https://doi.org/10.1111/j.1699-0463.1998.tb01359.x
Abstract
In order to establish a large panel of normal and tumour DNA from primary breast cancer patients, we looked for a source of easily accessible, good quality breast tumour DNA. Following routine hormone receptor analysis at the hospital the leftover pellets contained the nuclei from the tumour tissue. We collected 670 pellets over a period of 2 1/4 years and isolated a large amount of DNA (on average 400 μg per pellet). To control the quality of this tumour DNA, we analysed 41 pellets and matching normal DNA for loss of heterozygosity (LOH), with 11 microsatellite markers along chromosome 17. This chromosome is well described for breast cancer. LOH is a sensitive method, requiring good quality and pure tumour DNA. Contamination with normal DNA will blur the results. We found a high rate of LOH, ranging from 33 to 74%, which is in agreement with other reports, and therefore recommend this rich source of breast tumour DNA for molecular biological analysis.Keywords
This publication has 16 references indexed in Scilit:
- p53 activates expression of HIC-1, a new candidate tumour suppressor gene on 17p13.3Nature Medicine, 1995
- Evidence for a second tumor suppressor gene on 17p linked to high S-phase index in primary human breast carcinomasCancer Genetics and Cytogenetics, 1994
- Integrated human genome–wide maps constructed using the CEPH reference panelNature Genetics, 1994
- Automated construction of genetic linkage maps using an expert system (MultiMap): a human genome linkage mapNature Genetics, 1994
- A Comprehensive Genetic Linkage Map of the Human GenomeScience, 1992
- Determination of Estrogen Receptors in Paraffin-Embedded Tissue: Techniques and the value in breast cancer treatmentActa Oncologica, 1992
- Proceedings of the Second International Workshop on Human Chromosome 17 Mapping 1991Cytogenetic and Genome Research, 1991
- Evidence implicating at least two genes on chromosome 17p in breast carcinogenesisThe Lancet, 1990
- At least four different chromosomal regions are involved in loss of heterozygosity in human breast carcinomaGenomics, 1989
- Revision of the standards for the assessment of hormone receptors in human breast cancer; report of the second E.O.R.T.C. workshop, held on 16–17 March, 1979, in the Netherlands Cancer InstitutePublished by Elsevier ,1980