A Method for Rapid Determination of Lorazepam by High-Performance Liquid Chromatography
- 1 February 1993
- journal article
- Published by Wolters Kluwer Health in Therapeutic Drug Monitoring
- Vol. 15 (1) , 35-38
- https://doi.org/10.1097/00007691-199302000-00006
Abstract
A new sensitive method using solid-phase column extraction and high-performance liquid chromatography (HPLC) has been developed for the determination of lorazepam and its glucuronide metabolite in human plasma. Extraction was performed with a C2-Bond Elut column followed by reverse-phase HPLC of the sample. The detection limit of lorazepam in plasma was 0.5 μg/L. Standard curves over the concentration range from 2.5 to 75 μg/L had good linearity. Intra-assay variability ranged from 2.0 to 6.2% and interassay variability from 4.8 to 8% at the concentration range of 5–50 μg/L.Keywords
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