The Hardening Response of Three Clones of Dianthus and the Corresponding Complement of Peroxidase Isoenzymes1

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Abstract
The stems of 3 clones of Dianthus were studied at weekly intervals from August through November and the cold-hardiness as well as the peroxidase isoenzyme complement was determined. The hardening patterns showed indications of the typical plateau, this occurring at −10 to −14°G in November. The time of initiation of hardening correlated well with the winter-hardiness of the clones, the hardiest clone undergoing hardening 2 to 3 weeks before the tender clone. As determined by acrylamide gel electrophoresis of the stem extracts, the 2 winter hardy clones showed a gradual synthesis of 2 to 4 new peroxidase isoenzymes during the hardening period, whereas the tender clone showed only a relatively weak initiation of one isoenzyme. The formation of the new isoenzyme preceded the period of hardening by several weeks to more than a month, depending on the specific isoenzyme and the clone.

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