Initiation and termination of phage f1 plus-strand synthesis.

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Abstract
The origin of DNA replication of bacteriophage f1 contains a nucleotide sequence that is used both for the initation of viral (plus) strand synthesis and for its termination. With chimeric plasmids containing 2 f1 functional origins in the same orientation, synthesis of chimeric plus-strand DNA is initiated, after f1 infection, at either 1 of the 2 f1 origins and is terminated at the other. Thus, the chimeric plasmids segregate into 2 replicons, each of them containing only 1 f1 origin. This system was used to test several fragments of the f1 origin varying in size or in nucleotide sequence for their ability to function in either initiation or termination of viral strand synthesis. Evidently, the f1 origin is composed of 2 overlapping but distinct domains (signals), 1 for initiation and the other for termination of plus-strand synthesis.