Modeling fermentations with recombinant microorganisms: Formulation of a structured model
- 15 April 1991
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 37 (9) , 802-808
- https://doi.org/10.1002/bit.260370903
Abstract
A simple structured model is described and compared with experimental data for fermentations with recombinant. Escherichia coli. The model is a so-called compartment, model, where the different biomass components are lumped together in a few intracellular variables. The model is able to describe, in a biologically reasonable fashion, a majority of the observations that have been made through fermentations with recombinant microorganisms. The model is especially suited for description of dynamic changes in plasmid copy number, e.g., runaway plasmid replication.Keywords
This publication has 15 references indexed in Scilit:
- Cellular plasmid content and cloned-gene expression: Some useful equationsBiotechnology & Bioengineering, 1990
- Compartment model concept used in the design of fermentation with recombinant microorganismsBiotechnology & Bioengineering, 1989
- Use of culture fluorescence as a sensor for on-line discrimination of host and overproducing recombinantEscherichia coliBiotechnology & Bioengineering, 1989
- A novel structured kinetic modeling approach for the analysis of plasmid instability in recombinant bacterial culturesBiotechnology & Bioengineering, 1989
- Mathematical Modelling and Response Characteristics of Runaway Replication For Temperature Shift-upBiotechnology Progress, 1987
- Improvement of product yields by temperature‐shifting of Escherichia coli cultures containing plasmid pOU140Biotechnology & Bioengineering, 1987
- Simulations of host–plasmid interactions in Escherichia coli: Copy number, promoter strength, and ribosome binding site strength effects on metabolic activity and plasmid gene expressionBiotechnology & Bioengineering, 1987
- Mechanistically detailed model of cellular metabolism for glucose-limited growth ofEscherichia coli B/r-ABiotechnology & Bioengineering, 1986
- Continuous cultivation of recombinant Escherichia coli: Existence of an optimum dilution rate for maximum plasmid and gene product concentrationBiotechnology & Bioengineering, 1986
- Genetically structured models forlac promoter–operator function in the Escherichia coli chromosome and in multicopy plasmids: Lac operator functionBiotechnology & Bioengineering, 1984