Membrane lectins on human monocytes
- 12 November 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 193 (1) , 63-68
- https://doi.org/10.1016/0014-5793(85)80080-6
Abstract
Freshly isolated human monocytes, which do not contain cell‐surface mannose‐specific receptors, bind mannose 6‐phosphate and actively endocytose mannose 6‐phosphate‐bearing neoglycoproteins (6‐P‐Man‐F‐BSA). Three days after isolation, human monocytes endocytose very actively 6‐P‐Man‐F‐BSA as well as Man‐F‐BSA, and the endocytosed neoglycoproteins are rapidly degraded. These results were obtained in quantitative flow cytofluorometry by using a panel of fluoresceinylated sugar‐substituted serum albumins (neoglycoproteins). Thus, in contrast to mannose receptors which appear only after maturation, mannose 6‐phosphate receptors are already present on freshly isolated human monocytes.Keywords
This publication has 14 references indexed in Scilit:
- Tumoricidal activation of murine alveolar macrophages by muramyldipeptide substituted mannosylated serum albuminBiochemical and Biophysical Research Communications, 1984
- Uptake of neoglycoproteins via membrane lectin(s) of L1210 cells evidenced by quantitative flow cytofluorometry and drug targetingBiology of the Cell, 1984
- Sugar‐specific endocytosis of glycoproteins by Lewis lung carcinoma cellsJournal of Cellular Biochemistry, 1983
- Weak bases and ionophores rapidly and reversibly raise the pH of endocytic vesicles in cultured mouse fibroblasts.The Journal of cell biology, 1982
- Expression of a mannosyl-fucosyl receptor for endocytosis on cultured primary macrophages and their hybrids.The Journal of cell biology, 1982
- p-Isothiocyanatophenyl 6-phospho-.alpha.-D-mannopyranoside coupled to albumin. A model compound recognized by the fibroblast lysosomal enzyme uptake system. 1. Chemical synthesis and characterizationBiochemistry, 1980
- Phosphohexosyl recognition is a general characteristic of pinocytosis of lysosomal glycosidases by human fibroblasts.Journal of Clinical Investigation, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- The Facile Synthesis of 5′-Nucleotides by the Selective Phosphorylation of a Primary Hydroxyl Group of Nucleosides with Phosphoryl ChlorideBulletin of the Chemical Society of Japan, 1975
- The Glomerular Permeability Determined by Dextran Clearance Using Sephadex Gel FiltrationScandinavian Journal of Clinical and Laboratory Investigation, 1968