Characterization of amber mutations in bacteriophage Mu transposase: a functional analysis of the protein
- 31 August 1989
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 3 (9) , 1145-1158
- https://doi.org/10.1111/j.1365-2958.1989.tb00265.x
Abstract
We have characerized a series of amber mutations in the A gene of bacteriophage Mu encoding the phage transposase. We tested different activities of these mutant proteins either in a supo strain or in different sup bacteria. In conjunction with the results described in the accompanying paper by Betermier et al. (1989) we find that the C-terminus of the protein is not absolutely essential for global transposase function, but is essential for phage growth. Specific binding to Mu ends is defined by a more central domain. Our results also reinforce the previous findings (Betermier et al., 1987) that more than one protein may be specified by the A gene.This publication has 40 references indexed in Scilit:
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