Abstract
The amino acid sequence of the variable region of a rabbit anti-streptococcal A-variant antibody [Ab] L chain was determined. By using a combination of different cleavage methods, the sequence was established. Large peptides were sequenced in an extensively modified Beckman sequenator. L chain K29-213 belongs to a rare subgroup (.kappa.VI). Several of these L chains of Ab with different specificities were totally or partially sequenced. Comparison of these L chains reveals at least 4 germ-line-encoded variants within this subgroup.