Isolation of a Sperm Membrane Sialoglycoprotein Autoantigen from Rabbit Testes
Open Access
- 1 April 1979
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 122 (4) , 1248-1254
- https://doi.org/10.4049/jimmunol.122.4.1248
Abstract
A single rabbit sperm membrane autoantigen has been isolated from rabbit testes by immunoadsorbent chromatography, agarose chromatography, and electrophoresis. This rabbit sperm autoantigen (RSA-1) is located in the sperm plasma membrane and blocks the sperm cytotoxic (immobilizing) activity of autoantisera. RSA-1 is a sialoglycoprotein, approximately 40% carbohydrate and containing 0.2 µg sialic acid/µg protein. Its m.w. is estimated at 13,000 ± 1200 by SDS-PAGE. RSA-1 can self-aggregate into higher m.w. forms. Approximately 0.6 mg of RSA-1 can be isolated from 100 rabbit testes. The isolated RSA-1 shows a 300-fold increase in specific cytotoxic (immobilizing) inhibitory activity over the starting testis pellet material.Keywords
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