Quantitative analysis of tertiary and quaternary alkaloids in corydalis tuber by ion-pair high-performance liquid chromatography and its application to an oriental pharmaceutical preparation.

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Abstract
A new, simple and precise method using ion-pair high-performance liquid chromatography was developed for the determination of alkaloids in Corydalis Tuber and Anchusan-to dry extract, i.e., tetrahydrocolumbamine, glaucine, tetrahydropalmatine, cordyaline, columbamine, coptisine, palmatine, berberine and dehydrocorydaline. Reversed-phase systems with an octadecylsilane chemically bonded silica gel column using a mixture of sodium dodecyl sulfate, 0.05 M tartaric acid and acetonitrile (0.5:62:38) and a mixture of sodium dodecyl sulfate, Britton-Robinson buffer (pH 3.0) and acetonitrile (0.5:56:34) as the mobile phases were suitable for the separation of tertiary alkaloids and quaternary alkaloids, respectively, in Corydalis Tuber.