Genetical Studies on Copper Resistance of Saccharomyces cerevisiae

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Abstract
Copper resistant strains were obtained through repeated subculture of haploid strains in copper-containing media. Resistance was controlled by a dominant gene which segregated independently of mating type, galactose fermentability and growth type. Resistant clones having a genotype Rr and those having a lower level of resistance were obtained from a copper culture of a sensitive diploid strain, and the latter genotype was represented by R''r. Genetical analysis of resistant cells isolated from a copper culture inoculated by R''r seems to show that R, R'' and r are multiple alleles, and that the mutation rate from R'' to R is much higher than that from r to R'' and from r to R.

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