LABELING OF HUMAN-PLATELETS WITH [IN-111] 8-HYDROXYQUINOLINE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 20  (6) , 524-531
Abstract
The factors influencing the labeling of human platelets in the presence of autologous plasma were evaluated. The labeling efficiency was dependent on the time and temperature of incubation, the platelet concentration, the concentration of citrate ions (in ACD [acid-citrate-dextrose] anticoagulant), and the concentration of 8-hydroxyquinoline in the suspending medium. Contrary to what was expected, unsaturated transferrin did not interfere with the transfer of In-111 from the [111In] 8-hydroxyquinoline complex to the platelets. A protocol was established by which human platelets can be labeled with In-111 in plasma with a labeling efficiency of 55.5 .+-. 9.3 (mean .+-. 1 SD%).

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