Transfer RNA, a Possible Supplier of Free Cytokinins, Ribosyl-cis-zeatin and Ribosyl-2-methylthiozeatin: Quantitative Comparison between Free and Transfer Cytokinins in Various Tissues of the Hop Plant

Abstract
Cytokinins in both free pool and tRNAs have been identified and quantified in the unfertilized cone, fertilized cone, seed, cotyledon, epicotyl and root of the hop plant (Humulus lupulus L. cv. Shinshu-wase) on the basis of combined gas chromatography-mass spectrometry and combined gas chromatography-selected ion current monitoring analysis. Based on the quantitative comparison between free pool cytokinins and tRNA cytokinins, it is suggested that ribosyl-cis-zeatin and ribosyl-2-methylthiozeatin might be derived from tRNA catabolism and that the level of ribosyl-trans-zeatin might be controled independently of the tRNA catabolism.

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