Specific binding of nucleotides and NAD+ to Clostridium difficile toxin A
- 24 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 298 (2-3) , 185-187
- https://doi.org/10.1016/0014-5793(92)80052-i
Abstract
Binding of nucleotides, a tetrapolyphosphate, and NAD+ to purified toxin A of Clostridium difficile was determined by monitoring changes in intrinsic fluorescence following excitation at 280 nm, and recording emissions at 340 nm. Binding was specific for concentration over the range 5 to 100 μM for ATP, GTP, and their respective non‐hydrolysable analogues AMP‐PNP and Gpp(NH)p, tetrapolyphosphate and NAD+.Keywords
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