In vitro photosensitization II. An electron microscopy study of cellular destruction with mono‐L‐aspartyl chlorin e6and photofrin II

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Abstract
Primary sites of subcellular destruction with photofrin II (PfII) and mono‐L‐aspartyl chlorin e6(MACE) were determined by transmission electron microscopy (TEM).Potorous tridactylus(PTK2) cells were grown in Rose chambers and incubated for 24 hr with a sensitizer concentration sufficient to provide 100% mortality. Cells were irradiated with laser light and fixed and processed for electron microscopy at various times post‐irradiation. The results indicate that PfII initially destroys mitochondria, whereas MACE destroys lysosomes. Both conclusions are consistent with fluorescence subcellular localization studies.