Induction of Anti‐Phagocytic Surface Properties of Staphylococcus aureus from Bovine Mastitis by Growth in Milk Whey
- 12 January 1991
- journal article
- Published by Wiley in Journal of Veterinary Medicine, Series B
- Vol. 38 (1-10) , 401-410
- https://doi.org/10.1111/j.1439-0450.1991.tb00889.x
Abstract
Summary: The respiratory burst activity of bovine polymorphonuclear (PMN) cells in response to milk whey‐ and TSB‐grown S. aureus strains isolated from bovine mastitis was studied in whole blood chemiluminescence (CL) and in a CL system with purified bovine neutrophils. In both cases milk whey‐grown S. aureus strains elicited significantly less CL than homologous strains grown in TSB. Ingestion of milk whey‐grown S. aureus strains by bovine neutrophils was also considerably lower than that of the corresponding homologous organisms grown in TSB. Binding of complement factor C3 to serum‐opsonized milk whey‐grown S. aureus strains was lower compared with TSB‐grown homologous organisms. Moreover, 5 of 6 S. aureus strains grown in milk whey were significantly more resistant to in vivo clearance from the peritoneal cavity of mice compared with homologous bacteria grown in TSB.S. aureus strains grown in TSB exhibited hydrophobic surface properties, whereas homologous strains grown in milk whey were hydrophilic.Keywords
This publication has 33 references indexed in Scilit:
- Effects of exogenous oestradiol on the number and functional capacity of circulating mononuclear and polymorphonuclear leukocytes in the sowVeterinary Immunology and Immunopathology, 1990
- A Rapid Technique for the Isolation of Highly Purified, Functionally Intact Bovine Neutrophilic GranulocytesVeterinary Immunology and Immunopathology, 1988
- Effect of milk on surface properties ofStaphylococcus aureusfrom bovine mastitisFEMS Microbiology Letters, 1987
- The Use of Monoclonal Antibodies in Enzyme Linked Immunosorbent Assays for Detection of Antibodies to Bovine Viral Diarrhoea VirusJournal of Veterinary Medicine, Series B, 1987
- Evaluation and use of the drug inhibition method of measuring intracellular killing in differentiating between staphylococci grownin vivoandin vitroEpidemiology and Infection, 1983
- Encapsulation of Staphylococcus aureus isolated from bovine milkVeterinary Microbiology, 1983
- A new test based on ‘salting out’ to measure relative hydrophobicity of bacterial cellsBiochimica et Biophysica Acta (BBA) - General Subjects, 1981
- Degranulating Stimuli Decrease the Negative Surface Charge and Increase the Adhesiveness of Human NeutrophilsJournal of Clinical Investigation, 1980
- Phagocytosis as a Surface PhenomenonAnnual Review of Microbiology, 1978
- The Interaction Of Staphylococci Grown In Vivo And In Vitro With PolyMorphonuclear LeucocytesJournal of Medical Microbiology, 1970