DETECTION OF MURINE S180 CELLS EXPRESSING A MULTIDRUG RESISTANCE PHENOTYPE USING DIFFERENT INVITRO TEST SYSTEMS AND A MONOCLONAL-ANTIBODY

  • 1 July 1987
    • journal article
    • research article
    • Vol. 37-2  (7) , 862-867
Abstract
Doxorubicin (adriamycin) preconditioned S180 cells were more resistant to doxorubicin. The resistance was detected by three different methods (short-term test, colony assay, tissue culture assay). The doxorubicin-resistant S180 cells express the pleitrop drug resistance phenotype. There exists a multidrug resistance to doxorubicin, dactinomycin (actinomycin D), vincristine and colchicine. In addition, collateral sensitivity was found to fluorouracil (5-fluorouracil) and methotrexate. This multidrug phenotype is in accordance with the pleiotropic phenotype of colchicine-resistant CHO cells. Resistent S180 cells express a glycoprotein of Mr 170kd determined by indirect immunofluorescene using a monoclonal antibody (Mab 265/F4) to the P-glycoprotein of colchicine-resistant CHO-cell. The P-glycoprotein could be an important prognostic factors for tumors with multidrug resistance.