Toward an understanding of the formylation of initiator tRNA methionine in prokaryotic protein synthesis. II. A two-state model for the 70S ribosome
- 6 April 1976
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 15 (7) , 1362-1369
- https://doi.org/10.1021/bi00652a002
Abstract
The 70S ribosomes [Escherichia coli] can select the proper initiator tRNA between Met-tRNAfMet and fMet-tRNAfMet. Experiments on binding and on formation of aminoacylpuromycin, as a function of Mg, K or initiation factors, suggest a 2-state equilibrium for 70S particles, involving a minor, active conformation and a major one which is not readily active. The formyl group would act as a specific trigger to select the active conformation. Experimental results are interpreted following this simple model and equilibrium parameters, together with kinetic constants of the peptidyltransferase activity, are presented.Keywords
This publication has 2 references indexed in Scilit:
- Toward an understanding of the formylation of initiator tRNA methionine in prokaryotic protein synthesis. I. In vitro studies of the 30S and 70S ribosomal-tRNA complexBiochemistry, 1976
- Inhibitors of Ribosome FunctionsAnnual Review of Microbiology, 1971