Effect of Benzoic Acid on the Metabolic Conjugations of p-Aminobenzoic Acid in the Rat.

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Abstract
The rapid excretion of P-H2NC6H4CO2H (I) and its acetic acid, glycine, and glucuronic acid conjugates were detd. by the countercurrent distribution technique of Craig (CA 39, 13725) by using 1-pentanol and 0.1 [image] NaOAc buffers of pH 3.4 after administering I to white rats. After administering I mixed with II the amt. of glycine and glucuronic acid conjugates decreased whereas the amt. of P-ACNHC6H4CO2H increased and was the only metabolite of I excreted in significant amts. after administering NaOBz (H). Only a small fraction of the administered I was excreted as such and this was unaffected by administering II. The appreciable amts. of I glucuronides excreted made necessary the detn. of these conjugates in addn. to glucine and acetic acid conjugates; if this is not done the amts. of I and p-H2NC6H4CONHCH2-CO2H are estd. too high. The amt. of acetic acid conjugates increased from 53 to 85% after administering II, due not to any activation of the acetylation process but to the inhibition of the glycine conjugation leading to diminished excretion of total I.