Immunological Comparisons of Two Synthetic Human Parathyroid Hormone-(l-34) Peptides

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Abstract
The immunological properties of human parathyroid hormone-(1-34) [PTH-(1-34)] synthesized in accord with the sequence reported by Brewer et al. and the different sequence found by Niall et al. were compared with those of highly purified native human hormone-(1-84). Analyses were performed by radioimmunoassay using 7 anti-bovine PTH antisera and bovine PTH-(1-34) as tracer. Whereas human PTH-(1-34) synthesized in accord with the sequence of Niall et al. was immunologically indistinguishable from native human hormone in all 7 assay systems, striking differences were seen between human PTH-(1-34) synthesized in accord with sequence of Brewer et al. and the native hormone. In none of the 7 assay systems did human PTH-(1-34) synthesized in accord with the sequence of Brewer et at. give the same displacement slopes that the reference preparation gave. The observation that immunologic probes easily discriminate between the 2 different human PTH-(1-34) peptides suggests that similar immunologic approaches will be of value in exploring several important issues, particularly those relating to the sequence and conformational properties of human PTH and its synthetic peptides and to the question of the existence of isohormonal forms.

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