Forssman Antigen and Antibody: Preparation of Water Soluble Antigen and Measurement of Antibody Concentration by Precipitin Analysis, by C′1a Fixation and by Hemolytic Activity

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Abstract
Summary: Purified fractions of rabbit antisera containing 19 S Forssman antibody were analyzed for antibody content on a molecular basis by precipitin analysis, by C′1a fixation and transfer and by direct hemolysis with complement. We have found that the C′1a fixation and transfer test detects all antibody molecules which react with water soluble Forssman antigen, while direct hemolytic assay detects about one third of these molecules. Possible reasons for this paradox are discussed.