Abstract
Recent advances in the studies of sex-related differences of drug metabolism in the rat are described. Experiments with novel substrates provided new insights into the sex differences of drug metabolism. One of each form of cytochrome P-450, P-450-male and P-450-female, from liver microsomes of male and female rats, respectively, were purified to electrophoretic homogeneity. These forms of cytochrome P-450 are distinguishable from each other in their catalytic, physical and immunochemical properties. The use of antibodies to the 2 forms of cytochrome P-450 shows that P-450-male and P-450-female, present in rat-liver-microsomes, are dependent on sex hormones. Evidence for the involvement of multiple forms of cytochrome P-450 in the occurrence of the sex difference was presented.

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