Regulatory interactions in Arabidopsis thaliana acetohydroxyacid synthase
- 23 January 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 512 (1-3) , 180-184
- https://doi.org/10.1016/s0014-5793(02)02253-6
Abstract
Acetohydroxyacid synthase (AHAS; EC 4.1.3.18) contains catalytic and regulatory subunits, the latter being required for sensitivity to feedback regulation by leucine, valine and isoleucine. The regulatory subunit of Arabidopsis thaliana AHAS possesses a sequence repeat and we have suggested previously that one repeat binds leucine while the second binds valine or isoleucine, with synergy between the two sites. We have mutated four residues in each repeat, based on a model of the regulatory subunit. The data confirm that there are separate leucine and valine/isoleucine sites, and suggest a complex pathway for regulatory signal transmission to the catalytic subunit.Keywords
This publication has 10 references indexed in Scilit:
- Identification of the Regulatory Subunit ofArabidopsis thalianaAcetohydroxyacid Synthase and Reconstitution with Its Catalytic SubunitBiochemistry, 2001
- Acetohydroxyacid synthase: A proposed structure for regulatory subunits supported by evidence from mutagenesisJournal of Molecular Biology, 2001
- Evidence for Two Distinct Effector-Binding Sites in Threonine Deaminase by Site-Directed Mutagenesis, Kinetic, and Binding ExperimentsBiochemistry, 2000
- Mutations in Two Distinct Regions of Acetolactate Synthase Regulatory Subunit from Streptomyces cinnamonensis Result in the Lack of Sensitivity to End-Product InhibitionBiochemical and Biophysical Research Communications, 1999
- Expression, Purification, Characterization, and Reconstitution of the Large and Small Subunits of Yeast Acetohydroxyacid SynthaseBiochemistry, 1999
- Cloning and functional expression of the small subunit of acetolactate synthase from Nicotiana plumbaginifoliaPlant Molecular Biology, 1999
- An improved PCR-based method for site directed mutagenesis using megaprimersMolecular and Cellular Probes, 1998
- Biosynthesis of 2-aceto-2-hydroxy acids: acetolactate synthases and acetohydroxyacid synthasesBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1998
- Identification of an acetolactate synthase small subunit gene in two eukaryotesGene, 1997
- Acetolactate Synthase from Barley (Hordeum vulgare L.): Purification and Partial CharacterizationZeitschrift für Naturforschung C, 1988