Genetic mosaic with dual binary transcriptional systems in Drosophila
Top Cited Papers
- 2 April 2006
- journal article
- research article
- Published by Springer Nature in Nature Neuroscience
- Vol. 9 (5) , 703-709
- https://doi.org/10.1038/nn1681
Abstract
MARCM (mosaic analysis with a repressible cell marker) involves specific labeling of GAL80-minus and GAL4-positive homozygous cells in otherwise heterozygous tissues. Here we demonstrate how the concurrent use of two independent binary transcriptional systems may facilitate complex MARCM studies in the Drosophila nervous system. By fusing LexA with the VP16 acidic activation domain (VP16) or the GAL4 activation domain (GAD), we obtained both GAL80-insensitive and GAL80-suppressible transcriptional factors. LexA::VP16 can mediate MARCM-independent binary transgene induction in mosaic organisms. The incorporation of LexA::GAD into MARCM, which we call dual-expression-control MARCM, permits the induction of distinct transgenes in different patterns among GAL80-minus cells in mosaic tissues. Lineage analysis with dual-expression-control MARCM suggested the presence of neuroglioblasts in the developing optic lobes but did not indicate the production of glia by postembryonic mushroom body neuronal precursors. In addition, dual-expression-control MARCM with a ubiquitous LexA::GAD driver revealed many unidentified cells in the GAL4-GH146-positive projection neuron lineages.Keywords
This publication has 39 references indexed in Scilit:
- Mosaic Analysis with Double Markers in MiceCell, 2005
- Genetic mosaic analysis in the nervous systemCurrent Opinion in Neurobiology, 2004
- Transmembrane/Juxtamembrane Domain-Dependent Dscam Distribution and Function during Mushroom Body Neuronal MorphogenesisNeuron, 2004
- TGF-β Signaling Activates Steroid Hormone Receptor Expression during Neuronal Remodeling in the Drosophila BrainCell, 2003
- Drosophila Dscam Is Required for Divergent Segregation of Sister Branches and Suppresses Ectopic Bifurcation of AxonsNeuron, 2002
- Target neuron prespecification in the olfactory map of DrosophilaNature, 2001
- Mosaic analysis with a repressible cell marker (MARCM) for Drosophila neural developmentTrends in Neurosciences, 2001
- Cell-Autonomous Requirement of the USP/EcR-B Ecdysone Receptor for Mushroom Body Neuronal Remodeling in DrosophilaNeuron, 2000
- Essential Roles of Drosophila RhoA in the Regulation of Neuroblast Proliferation and Dendritic but Not Axonal MorphogenesisNeuron, 2000
- Mosaic Analysis with a Repressible Cell Marker for Studies of Gene Function in Neuronal MorphogenesisNeuron, 1999