Physical Analysis of Autographa californica Nuclear Polyhedrosis Virus Transcripts for Polyhedrin and 10,000-Molecular-Weight Protein

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Abstract
In Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells, polyhedrin, the major structural polypeptide of the viral occlusions and a low MW viral protein with a MW of .apprx. 10,000 (10K) accumulated to high levels late in infection. Two polyadenylated RNA 1200 and 630 bases in size were the most abundant viral transcripts present in the cytoplasm at 48 h postinfection. Evidence is presented that these RNA were the transcripts for polyhedrin and 10K proteins. The 630-base RNA and 5 other major polyadenylated RNA present at 48 h postinfection mapped within or near A. californica nuclear polyhedrosis virus EcoRI-P. The DNA sequences that code for polyhedrin mRNA were examined by S1 nuclease analysis. The polyhedrin gene contained no detectable introns and mapped at 3990-5200 base pairs to the right of the origin of the circular A. californica nuclear polyhedrosis virus physical map. Heterogeneity at the 5'' end of polyhedrin mRNA was observed by using S1 nuclease analysis with 5'' end-labeled DNA probes.