The effects on plasma cholesterol level of 4 fats each supplied at 3 levels in a high and a low protein diet were studied in the cholesterol-fed chicken. On the low protein diet a marked hypercholesterolemia was observed which was not affected by type of fat studied or level of supplementation. On the high protein diet, plasma cholesterol levels were essentially normal regardless of source and level of dietary fat. Since the fats used represented a wide range in the product EFA x TSFA, a lack of relationship between this product and plasma cholesterol level in the growing chicken is indicated.