VII. Yeast sequencing reports. Physical, transcriptional and genetical mapping of a 24 kb DNA fragment located between the PMA1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae
- 1 April 1991
- Vol. 7 (3) , 275-280
- https://doi.org/10.1002/yea.320070309
Abstract
A physical map of a contiguous DNA fragment of 60 kb, extending from the centromere to TRP5 on the left arm of the chromosome VII of Saccharomyces cerevisiae, strain IL 125‐2B, was established. Within a 31 kb region from PMA1 towards TRP5, a total of 12 transcription products ranging from 0·6 to 3·6 kb were identified in cells grown exponentially on rich medium. Near 87% of the DNA investigated was transcribed and on average one transcript, of 2·3 kb average length, was detected every 2·7 kb of DNA. The physical and genetical distances between the markers CEN7, pma1, leu1, pdr1 and trp5 were compared. A recombination frequency of 1 cM corresponds to an average distance of 3·3 kb between alleles in this region of chromosome VII.Keywords
This publication has 28 references indexed in Scilit:
- VII. Yeast sequencing reports. The DNA sequencing of the 17 kbHindIII fragment spanning theLEU1 andATE1 loci on chromosome VII fromSaccharomyces cerevisiae reveals thePDR6 gene, a new member of the genetic network controlling pleiotropic drug resistanceYeast, 1991
- VII. Yeast sequencing reports. The YGL023 gene encodes a putative regulatory proteinYeast, 1991
- VII. Yeast sequencing reports. The YGL021 gene encodes a putative membrane protein with a putative leucine zipper motifYeast, 1991
- VII. Yeast sequencing reports. The YGL022 gene encodes a putative transport proteinYeast, 1991
- Cloning and functional analysis of the arginyl-tRNA-protein transferase gene ATE1 of Saccharomyces cerevisiae.Journal of Biological Chemistry, 1990
- The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growthGene, 1989
- Physical analysis of the COR region: a cluster of six genes in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1987
- Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome.Molecular and Cellular Biology, 1986
- [35] Transformation of nuclear respiratory deficient mutants of yeastPublished by Elsevier ,1983
- Construction of new yeast vectors and cloning of the nif (nitrogen fixation) gene cluster of Klebsiella pneumoniae in yeastCurrent Genetics, 1981