Excess Chromosome #4 in Ethylnitrosourea-Induced Neurogenic Tumor Lines of the Rat 2

Publisher Website
Google Scholar
Abstract
The chromosomes in 15 cell lines derived from separate tumors induced in rats by ethylnitrosourea (ENU) are described. Thirteen lines were neural (glioma or schwannoma) in origin and type. In 12 of these lines, excess chromosome #4 could be demonstrated by Giemsa banding. One to three extra #4 chromosomes were seen as numerical or structural abnormalities. Also noted were other changes that were not consistent among lines. The 12 lines produced tumors in newborn rats. The 13th neurogenic line lacked excess chromosome #4 and did not produce tumors. The remaining 2 lines were nonneurogenic and lacked excess chromosome #4 but produced tumors. Control studies included chromosome analyses of bone marrow preparations from ENU-treated rats with tumors, cell lines from brains of normal rat embryos, and 2 established nonneurogenic rat tumor lines. No excess chromosome #4 was seen. These results suggest that nondisjunction and/or rearrangement of chromosome #4 is associated with the oncogenic process in neurogenic tumors induced by ENU.

This publication has 1 reference indexed in Scilit: