Effects of Colchicine, Griseofulvin and Caffeine on Cell Shape and Septum Formation of Cultured Carrot Cells in Suspension

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Abstract
Colchicine at the concentration of 10-4 M completely inhibited phragmoplast formation of cultured carrot [Daucus carota] cells and produced large spherical cells, wherea griseofulvin and caffeine produced large but irregular cells. In the presence of griseofulvin or a lower concentration of colchicine (10-5 M), septum formation was incomplete and highly irregular cross walls were formed. Caffeine also inhibited normal septum formation, but it did not induce intricate cross walls. Inhibition of cytokinesis by 10-4 M colchicine could be the result of the loss of cytoplasmic microtubules in the phragmoplast region. In cells treated with 10-5 M colchicine or 5 .times. 10-5-10-3 M griseofulvin, a considerable number of microtubules remained but the array of vesicles in the phragmoplast region was markedly disordered. Caffeine seemed to inhibit fusion of the vesicles aligned in the phragmoplast region.

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