Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA.
- 1 December 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (24) , 9006-9010
- https://doi.org/10.1073/pnas.84.24.9006
Abstract
Crude protein extracts of induced and uninduced octopine wild-type strain of Agrobacterium tumefaciens, as well as several mutants of the virulence loci virA, -B, -G, -C, -D, and -E, were probed with single- and double-stranded synthetic oligodeoxynucleotides of different sequence and length in an electrophoretic retardation assay. Four complexes involving sequence-nonspecific, single-stranded-DNA-binding proteins were recognized. One inducible complex is determined by the virE locus, two Ti-plasmid-dependent complexes are constitutively expressed, and a fourth one is controlled by chromosomal genes. The protein-DNA complexes were characterized by sucrose density gradient centrifugation and by determination of the length of single-stranded DNA required for their formation. It is hypothesized that the single-stranded-DNA-binding proteins are involved in the production of T-DNA intermediates or have a carrier or protective function during T-DNA transfer.This publication has 29 references indexed in Scilit:
- Virulence genes A, G , and D mediate the double-stranded border cleavage of T-DNA from the Agrobacterium Ti plasmidProceedings of the National Academy of Sciences, 1987
- Sequence comparison of single-stranded DNA binding proteins and its structural implicationsJournal of Molecular Biology, 1987
- Characterization of thevirEoperon of theAgrobacteriumTi plasmid pTiA6Nucleic Acids Research, 1987
- virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciensCell, 1986
- SINGLE-STRANDED DNA BINDING PROTEINS REQUIRED FOR DNA REPLICATIONAnnual Review of Biochemistry, 1986
- A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genesNature, 1986
- Nucleotide sequence of the virulence genevirGof theAgrobacterium tumefaciensoctopine Ti plasmid: significant homology betweenvirGand the regulatory genesompR,phoBanddyeofE. coliNucleic Acids Research, 1986
- A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosomeCell, 1984
- Processing of plasmid DNA during bacterial conjugation.1984
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982