Characterization of Blood‐Group‐H‐Active Ceramide Tetrasaccharide from Hog‐Stomach Mucosa

Abstract
A fucose‐containing ceramide tetrasaccharide exhibiting blood‐group‐H activity was isolated from a lipid extract of hog stomach mucosa by column fractionations on DEAE‐cellulose and Florisil followed by purification on thin‐layer plates.By partial acid hydrolysis, sequential degradation with specific glycosidases and methylation analysis, the structure of this glycolipid was identified as: Fucα1 →, 2Galα1 → 3Galβ1 → 4Glc1 → 1Cer; where Fuc = fucose; Gal = glucose; Gal = glucose and Cer = ceramide.