Cellular mechanisms in proximal tubular reabsorption of inorganic phosphate
- 1 May 1991
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 260 (5) , C885-C899
- https://doi.org/10.1152/ajpcell.1991.260.5.c885
Abstract
Filtered inorganic phosphate (Pi) is largely reabsorbed in the proximal tubule. Na-Pi cotransport, with a stoichiometry of at least 2:1, mediates uphill transport at the apical membrane; at the basolateral membrane different types of transport systems can be involved in efflux and uptake of Pi from the interstitium. Regulation of transcellular Pi flux involves alteration of the apical Na-Pi cotransport; at least three different cellular control/sensing systems seem to participate in this regulation and are exemplified by parathyroid hormone (PTH)-dependent inhibition, Pi deprivation-dependent increase, and insulin-like growth factor I (IGF-I)-dependent increase in Na-Pi cotransport. For PTH inhibition, recent evidence suggests a role of the phospholipase C/protein kinase C-dependent regulatory cascade in inhibition of Na-Pi cotransport, at least at low PTH concentrations. In addition, an endocytic mechanism seems to be involved in this PTH action. Little is known of the cellular mechanisms in Pi deprivation-dependent and/or IGF-I-dependent increases in Na-Pi cotransport; they are dependent on de novo protein synthesis. Recent experiments involving an expression in Xenopus laevis oocytes led to the identification of an approximately 50 kDa membrane protein that is a good candidate for being involved in brush-border membrane Na-Pi cotransport activity.Keywords
This publication has 59 references indexed in Scilit:
- Dissociation of cAMP accumulation and phosphate uptake in opossum kidney (OK) cells with parathyroid hormone (PTH) and parathyroid hormone related protein (PTHrP)Peptides, 1990
- Parathyroid hormone inhibition of Na+/phosphate cotransport in OK cells: Generation of second messengers in the regulatory cascadeBiochemical and Biophysical Research Communications, 1989
- Atrial natriuretic factor inhibits phosphate uptake in opossum kidney cells: As a model of renal proximal tubulesBiochemical and Biophysical Research Communications, 1988
- Parathyroid hormone regulates phosphate transport in OK cells via an irreversible inactivation of a membrane proteinFEBS Letters, 1987
- Phorbol esters inhibit phosphate uptake in opossum kidney cells: A model of proximal renal tubular cellsBiochemical and Biophysical Research Communications, 1987
- Stimulation of inositol trisphosphate and diacylglycerol production in renal tubular cells by parathyroid hormone.Journal of Clinical Investigation, 1987
- Transport studies in plasma membrane vesicles isolated from renal cortexKidney International, 1986
- Parathyroid hormone stimulation of renal phosphoinositide metabolism is a cyclic nucleotide-independent effectBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- Parathyroid hormone acutely increases polyphosphoinositides of the rabbit kidney cortex by a cycloheximide-sensitive process.Journal of Clinical Investigation, 1980
- Distribution of membrane-bound cyclic AMP-dependent protein kinase in plasma membranes of cells of the kidney cortexThe Journal of Membrane Biology, 1975