Modified fluorimetric procedure for the simultaneous determination of thiamin and riboflavin in cowpea

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Abstract
The fluorimetric methods for the assay of thiamin and riboflavin have been modified so that the same sample extract may be used for the simultaneous analysis of the two B vitamins. The procedure eliminates acid hydrolysis and specific pH adjustment for either vitamin; the sample extract is obtained after enzymic hydrolysis only with Clarase and has a pH of 4.5–4.8. A standard additions method for thiamin determination is introduced. A cowpea sample analysed by this procedure gave mean recoveries and standard errors of 103.9 ± 1 and 100.5 ± 2% for added thiamin and riboflavin, respectively; the detection limits for the aqueous solutions taken for fluorescence measurement according to the described procedures for these two vitamins are 0.0025 and 0.0020 µg ml–1, respectively.

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